FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005067841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45997348
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43828609.52850586081615No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA5321411.1568949583789048No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT2994090.6509266577716611No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA1943170.4224526161812633No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT1790280.389213743366248No Hit
GATGAAACCAATTTAAATAGTTGCTAATGTCCTGACTGGCCTGGCAAGTG1765880.3839090897153462No Hit
GGAACTTAGGGGCTTTCCCTGGTTTCTGATGAAACCAATTTAAATAGTTG1415330.3076981742512634No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1378370.2996629283931761No Hit
GGCCTGGCAAGTGATGGTGACTCTGTCCCCTACAGATGTAGACAGGGAGG1202580.2614455076844865No Hit
GTTGCTAATGTCCTGACTGGCCTGGCAAGTGATGGTGACTCTGTCCCCTA1110450.24141609207556924No Hit
GTCCTGACTGGCCTGGCAAGTGATGGTGACTCTGTCCCCTACAGATGTAG1095400.23814416431138596No Hit
GGTGACTCTGTCCCCTACAGATGTAGACAGGGAGGATGGAGACTGGGTCA991320.2155167728365557No Hit
GTGATGGTGACTCTGTCCCCTACAGATGTAGACAGGGAGGATGGAGACTG988810.2149710892027949No Hit
GATGTAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGC985370.21422321999955302No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG971950.21130566049155702No Hit
GATGCATTGTAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGATGAAA945140.2054770635907096No Hit
GGGGCTTTCCCTGGTTTCTGATGAAACCAATTTAAATAGTTGCTAATGTC940430.20445309151301505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG888770.1932220092340976No Hit
GATGGTGACTCTGTCCCCTACAGATGTAGACAGGGAGGATGGAGACTGGG883770.192134990043339No Hit
GGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGC879650.19123928623015396No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG875090.19024792472818217No Hit
GGCTTTCCCTGGTTTCTGATGAAACCAATTTAAATAGTTGCTAATGTCCT862750.18756516136539No Hit
AACCAATTTAAATAGTTGCTAATGTCCTGACTGGCCTGGCAAGTGATGGT828160.1800451626037223No Hit
GTGACTCTGTCCCCTACAGATGTAGACAGGGAGGATGGAGACTGGGTCAT823980.17913641456024812No Hit
GATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGATGAAACCAATTTAAAT807200.17548837815606239No Hit
GCCACTTCCACTGAATCTTGATGGGACCCCTGTTTCCAAATTGGATGCAT799700.17385784936992454No Hit
CAGGAACTTAGGGGCTTTCCCTGGTTTCTGATGAAACCAATTTAAATAGT762840.16584434389565242No Hit
CTAAAATTTGTCCCAGAGCCACTTCCACTGAATCTTGATGGGACCCCTGT759000.16500951315714985No Hit
CTGATGAAACCAATTTAAATAGTTGCTAATGTCCTGACTGGCCTGGCAAG758980.16500516508038682No Hit
GGCAAGTGATGGTGACTCTGTCCCCTACAGATGTAGACAGGGAGGATGGA730510.15881567780820757No Hit
GTCACATCTGGCACCTGAGAGCCAGAGCAGCAGGAGCCCCAGGAGCTGAG728890.15846348359040177No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG723920.15738298651478777No Hit
ACTGAATCTTGATGGGACCCCTGTTTCCAAATTGGATGCATTGTAGATCA723880.1573742903612617No Hit
GCCTGGCAAGTGATGGTGACTCTGTCCCCTACAGATGTAGACAGGGAGGA723800.15735689805420958No Hit
GTCCCAGAGCCACTTCCACTGAATCTTGATGGGACCCCTGTTTCCAAATT709260.15419584624748367No Hit
CCTGGTTTCTGATGAAACCAATTTAAATAGTTGCTAATGTCCTGACTGGC694570.151002183865035No Hit
GGATGCATTGTAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGATGAA689850.14997603774895893No Hit
GGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCC688080.1495912329554304No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG682860.14845638492027843No Hit
GGTGAAACTAAAATTTGTCCCAGAGCCACTTCCACTGAATCTTGATGGGA678310.14746719745668815No Hit
GGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAGCAGCAGGAGC667850.14519315330962124No Hit
GGCTGCAGGCTGCTGATGGTGAAACTAAAATTTGTCCCAGAGCCACTTCC662890.14411483027238875No Hit
GTCCCCTACAGATGTAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT645220.14027330445224798No Hit
AATGTCCTGACTGGCCTGGCAAGTGATGGTGACTCTGTCCCCTACAGATG638800.138877571811314No Hit
GACCCCTGTTTCCAAATTGGATGCATTGTAGATCAGGAACTTAGGGGCTT637790.13865799393478076No Hit
GTAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGATGAAACCAATTTA634120.13786012184876398No Hit
GGCTGCTGATGGTGAAACTAAAATTTGTCCCAGAGCCACTTCCACTGAAT612470.1331533287527794No Hit
GGTTTCTGATGAAACCAATTTAAATAGTTGCTAATGTCCTGACTGGCCTG609180.13243807012526027No Hit
GAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAGCAGC607140.13199456629543077No Hit
CCCTGGTTTCTGATGAAACCAATTTAAATAGTTGCTAATGTCCTGACTGG605850.13171411534421507No Hit
CTAATGTCCTGACTGGCCTGGCAAGTGATGGTGACTCTGTCCCCTACAGA602900.1310727740216675No Hit
GCCAGAGCAGCAGGAGCCCCAGGAGCTGAGCAGGGACCCTCATGTCCATG602420.1309684201793547No Hit
CAAATTGGATGCATTGTAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCT601790.1308314557613191No Hit
GACTGGCCTGGCAAGTGATGGTGACTCTGTCCCCTACAGATGTAGACAGG599390.130309686549755No Hit
GAATCTTGATGGGACCCCTGTTTCCAAATTGGATGCATTGTAGATCAGGA593400.12900743755922622No Hit
AATTTAAATAGTTGCTAATGTCCTGACTGGCCTGGCAAGTGATGGTGACT592380.1287856856443115No Hit
GTAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACC592260.12875959718373328No Hit
GTGAAACTAAAATTTGTCCCAGAGCCACTTCCACTGAATCTTGATGGGAC591480.12859002218997495No Hit
GTTGCAACGTCTTCAGGCTGCAGGCTGCTGATGGTGAAACTAAAATTTGT586420.12748995876892727No Hit
GCTAATGTCCTGACTGGCCTGGCAAGTGATGGTGACTCTGTCCCCTACAG579650.1260181347846402No Hit
CAAGTGATGGTGACTCTGTCCCCTACAGATGTAGACAGGGAGGATGGAGA570670.1240658483180378No Hit
GACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGA569750.12386583678693824No Hit
GGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGA565580.12295926278184559No Hit
CTGGCCTGGCAAGTGATGGTGACTCTGTCCCCTACAGATGTAGACAGGGA559310.12159614071663435No Hit
GATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG558400.1213983032239163No Hit
CCAAATTGGATGCATTGTAGATCAGGAACTTAGGGGCTTTCCCTGGTTTC553590.12035259076240655No Hit
AGGAACTTAGGGGCTTTCCCTGGTTTCTGATGAAACCAATTTAAATAGTT552950.12021345230598948No Hit
GCAACGTCTTCAGGCTGCAGGCTGCTGATGGTGAAACTAAAATTTGTCCC552520.12011996865558423No Hit
CCCCTGTTTCCAAATTGGATGCATTGTAGATCAGGAACTTAGGGGCTTTC551880.11998083019916714No Hit
CCTGACTGGCCTGGCAAGTGATGGTGACTCTGTCCCCTACAGATGTAGAC543900.11824594757071646No Hit
GAAACCAATTTAAATAGTTGCTAATGTCCTGACTGGCCTGGCAAGTGATG543490.11815681199707426No Hit
CTCTGTCCCCTACAGATGTAGACAGGGAGGATGGAGACTGGGTCATCTGG536700.11668063993602414No Hit
ATGGTGACTCTGTCCCCTACAGATGTAGACAGGGAGGATGGAGACTGGGT534020.11609799764977756No Hit
ATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGATGAAACCAATTTAAATA525920.11433702656074868No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG525830.11431746021531503No Hit
AGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGATGAAACCAATTTAAA519030.11283911411588338No Hit
ATTGTAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGATGAAACCAAT514110.11176948723217696No Hit
CTGGCAAGTGATGGTGACTCTGTCCCCTACAGATGTAGACAGGGAGGATG513890.11172165838778357No Hit
GGCACCTGAGAGCCAGAGCAGCAGGAGCCCCAGGAGCTGAGCAGGGACCC513290.11159121608489254No Hit
CTGGTTTCTGATGAAACCAATTTAAATAGTTGCTAATGTCCTGACTGGCC511170.11113031994801092No Hit
AGTTGCTAATGTCCTGACTGGCCTGGCAAGTGATGGTGACTCTGTCCCCT505400.10987589980187554No Hit
CTTTCCCTGGTTTCTGATGAAACCAATTTAAATAGTTGCTAATGTCCTGA504010.10957370846684465No Hit
AGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTG503360.10943239597204603No Hit
AAATTGGATGCATTGTAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTG500910.10889975656857434No Hit
CCCAGAGCCACTTCCACTGAATCTTGATGGGACCCCTGTTTCCAAATTGG500380.10878453253435393No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC495210.10766055469110958No Hit
GGATGTCACATCTGGCACCTGAGAGCCAGAGCAGCAGGAGCCCCAGGAGC493610.10731270855006685No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT490920.10672789222543873No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC488310.10616046820786276No Hit
GGGCTTTCCCTGGTTTCTGATGAAACCAATTTAAATAGTTGCTAATGTCC487930.10607785474936512No Hit
GATGGTGAAACTAAAATTTGTCCCAGAGCCACTTCCACTGAATCTTGATG485530.105556085537801No Hit
GACTCTGTCCCCTACAGATGTAGACAGGGAGGATGGAGACTGGGTCATCT479770.10430383943004715No Hit
TAAATAGTTGCTAATGTCCTGACTGGCCTGGCAAGTGATGGTGACTCTGT479200.10417991924230068No Hit
GCTTTCCCTGGTTTCTGATGAAACCAATTTAAATAGTTGCTAATGTCCTG478860.10410600193732908No Hit
AAACTGTTGACAGTAATATGTTGCAACGTCTTCAGGCTGCAGGCTGCTGA478420.10401034424854232No Hit
CTGACTGGCCTGGCAAGTGATGGTGACTCTGTCCCCTACAGATGTAGACA476800.10365815003073656No Hit
GACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAGCAGCAG470480.10228415777361773No Hit
AATAGTTGCTAATGTCCTGACTGGCCTGGCAAGTGATGGTGACTCTGTCC469650.1021037125879518No Hit
ATGCATTGTAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGATGAAAC465300.10115800589199186No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)