FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647569
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1471748.932797351734587No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA542073.290120170991321No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT240321.4586339024344352No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG120130.7291348647613545No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT117740.7146286437775898No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT91280.5540283897062885No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG78220.474760085920529No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG76850.46644480443611164No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG73900.4485396362762349No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG71500.4339727198071826No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA69350.4209231904703232No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC46760.28381208920536866No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC42190.2560742524288816No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT31940.1938613800089708No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA23030.1397817026176142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22900.13899266130887386No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT21910.1329838082653898No Hit
GGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTGGG21360.1296455565745653No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC18260.11082995613537279No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC18170.11028369676778334No Hit
GTGGAGACTGGCCTGGCTTCTGCAGGTACCAAGACAAATAGGCCTTCCCA17790.10797726832685005No Hit
GTGAAATCTGTCCCTGACCCGCTGCCACTGAACCTAACTGGAACTCCAGA17120.10391067081257294No Hit
GGCCTTCCCATCAGTATGCAGGAGGCTCTGACTAGATTTGCAGGAGATGG16790.10190771979807825No Hit
GGTACCAAGACAAATAGGCCTTCCCATCAGTATGCAGGAGGCTCTGACTA16580.10063311460703618No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)