FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8946507
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA2210212.4704725542605623No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1442131.6119475455616366No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT996871.1142561001740678No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT904651.011176764294713No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG389090.4349071654445696No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG349200.39031993156658795No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC348460.38949279310908713No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC320400.35812859700439514No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT318800.35634018952871777No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT265370.29661855738781623Clontech SMART CDS Primer II A (100% over 26bp)
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG244600.2734027928441793No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG193350.21611786588888823No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT152850.1708488016608046No Hit
GGCCTGGCCTCTGTTGATACCAAGAAGTGTATCCATTTCCCAAGTTATCT130270.1456099011603076No Hit
GGTGACGCTGGCTGTCTGTCCTGGGGTCACGGACACTGAGGGTGGCTGAG122390.1368019943425965No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC118560.1325209939476938No Hit
GCCTGGCCTCTGTTGATACCAAGAAGTGTATCCATTTCCCAAGTTATCTC116680.13041961516377285No Hit
CTCCAGAGTTGGAGGCAGAAAACCGCTCAGGGATCCCTGAGGGCCGCTTG108370.12113107383697347No Hit
GTATCCATTTCCCAAGTTATCTCCAGAGCAGGTGACGCTGGCTGTCTGTC102210.11424570505561556No Hit
GCCGCTTGGTATCTTGATAGATGACCAACAGAGGGGACTGGCCTGGCCTC102180.11421217241544661No Hit
GTTGATACCAAGAAGTGTATCCATTTCCCAAGTTATCTCCAGAGCAGGTG102170.11420099486872362No Hit
GTCACGGACACTGAGGGTGGCTGAGTCAGTTCATAGGAGGCCACGAAACC100690.11254671795372206No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA100470.11230081192581641No Hit
GCTTGGTATCTTGATAGATGACCAACAGAGGGGACTGGCCTGGCCTCTGT99310.11100421650595031No Hit
GGCAGAAAACCGCTCAGGGATCCCTGAGGGCCGCTTGGTATCTTGATAGA98330.10990881692709792No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98090.1096405558057463No Hit
GTCAGAGTGGCGGTGTCTCCAGAGTTGGAGGCAGAAAACCGCTCAGGGAT94690.10584018991993188No Hit
GCCTCATCGAGAGTCTGGGTCCCGCTGATGGTCAGAGTGGCGGTGTCTCC94590.10572841445270205No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG92220.10307933587935493No Hit
GGACTGGCCTGGCCTCTGTTGATACCAAGAAGTGTATCCATTTCCCAAGT90190.10081029389458925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)