FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354568
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT676512.016682923106641Clontech SMART CDS Primer II A (100% over 26bp)
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA194160.5787928579775399No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT150360.4482246298182061No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC130960.3903930401768574No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43880.13080670894135996Clontech SMART CDS Primer II A (100% over 25bp)
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT38260.11405343400402079No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)