FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212965
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG59900.18643215845799754No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA46400.1444148940309029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG34200.0140.332352
TAGTCGA41850.0139.103877
GTCGATA42050.0138.444419
TAAACCG22250.0137.175951
GACCTTA33650.0136.808962
ACACGAA20350.0136.44377
CTAGTCG42750.0136.007896
TCGCATG38150.0134.732038
GCGAAGT20050.0133.786794
GTACACG23350.0133.508045
TACACGG23450.0133.247886
TCTAGTC43950.0132.789255
CACGAAA21600.0132.575268
GCGACAC13800.0131.862264
GTCTCGA14700.0131.685
CGATGTA23950.0131.676911
CACGGCG24050.0131.428798
AGTTCGG29250.0131.363855
CAGTTCG38900.0131.204574
TCGGCTA31500.0131.184778
Produced by FastQC (version 0.11.5)