FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661707
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT924335.562532985658724No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA504363.035192124724756No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT223311.3438590557781847No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT104420.6283899628514533No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG77400.4657860862354194No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT63220.38045214950650147No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT62220.37443424141560455No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG58390.35138565342746947No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG42720.2570850336431152No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG40120.24143847260678325No Hit
TAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGT34750.20912230615866695No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG33380.2008777720741382No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA31380.18884195589234443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26760.1610392205124008No Hit
CCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGC25090.150989314000603No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC23770.1430456753206191No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC22670.13642597642063253No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT22540.13564364836881593No Hit
GCTGAAACCAGGCTAAATTATTGCTAATGTCCTGACTCGCCCGACAAGTG19270.11596508891158308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATC101600.0143.194442
GCGTTAT104350.0139.721831
TTATCCA107150.0135.33064
ATCCACC107500.0134.945256
CACGCTG61750.0134.569081
CACCTTC124050.0117.109949
ACGCTGC71500.0116.040332
GTTATCC126350.0115.364073
GGAAGTT38200.0114.863212
GAAGTTT38800.0113.263673
AAGTTTC39150.0112.412634
TTCTGGC40500.0108.6491858
CCACCTT137050.0105.631118
TCGTATC78100.0105.350769
CTCGTAT78700.0105.008148
CTGCTCG78750.0104.9477845
CGCTGCT79450.0104.3282853
GCTCGTA79550.0103.977257
TTTCTGG42350.0103.7318347
TGCTCGT80950.0102.095586
Produced by FastQC (version 0.11.5)