FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072192
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1978789.54921165606276No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA982654.742079884489468No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG229601.1080054357897338No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT197040.9508771387979491No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG149910.7234368243869295No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG136790.6601222280560874No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA96740.46684863178701586No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT84740.40893894002100195No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG64700.3122297547717586No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG60420.29157529804188026No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT44480.21465192414602507No Hit
GTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACT43930.21199772994008279No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC43210.20852314843412192No Hit
GCTGATACCAGGCTAAATAACTGCTAATACCCTGACTCGCCCGACAAGGG41120.19843721045154117No Hit
GGAGCTTAGGGGCTTCCCCTGGTTTTTGCTGATACCAGGCTAAATAACTG39800.19206714435727965No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37440.18067823830996357No Hit
GCTGAACCTTGATGGGACCCCACTTTGCAAAGTGGACGCAGCATAGATCA37380.1803886898511335No Hit
GCCCGACAAGGGATGGTGACTCTGTCTCCTGTAGATGCAGAGAATGAGGA35570.17165397800975973No Hit
GGGATGGTGACTCTGTCTCCTGTAGATGCAGAGAATGAGGATGGAGACTG34150.16480133115078138No Hit
GGGGCTTCCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTAATACC32450.15659745815059609No Hit
GCAAAGTGGACGCAGCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTT30190.1456911328679968No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAAGT30140.14544984248563841No Hit
GTGACTCTGTCTCCTGTAGATGCAGAGAATGAGGATGGAGACTGGGTCAT29110.14047926060905552No Hit
GGCTTCCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTAATACCCT28490.13748725986781146No Hit
GGCTAAATAACTGCTAATACCCTGACTCGCCCGACAAGGGATGGTGACTC28130.13574996911483106No Hit
GCAAAATCTTCAGACTGCAGGCAGCTGATGGTGAGAGTGAAATCTGTCCC27740.1338679041324356No Hit
GATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGCTGATACCAGGCTAAAT26870.1296694514793996No Hit
GGACGCAGCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGCTGAT26400.12740132188523073No Hit
GGCAGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAAC25730.12416803076162827No Hit
CCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTAATACCCTGACTCGC25710.1240715146086849No Hit
GTTGCAAAATCTTCAGACTGCAGGCAGCTGATGGTGAGAGTGAAATCTGT25610.12358893384396813No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG25360.12238248193217618No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT24620.11881138427327198No Hit
GACCCCACTTTGCAAAGTGGACGCAGCATAGATCAGGAGCTTAGGGGCTT24230.11692931929087652No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC23940.11552983507319785No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23470.11326170547902897Clontech SMART CDS Primer II A (100% over 26bp)
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC23460.11321344740255729No Hit
GAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTT23170.11181396318487861No Hit
GGTGACTCTGTCTCCTGTAGATGCAGAGAATGAGGATGGAGACTGGGTCA23120.11157267280252023No Hit
GGTTTTTGCTGATACCAGGCTAAATAACTGCTAATACCCTGACTCGCCCG23120.11157267280252023No Hit
GCTAAATAACTGCTAATACCCTGACTCGCCCGACAAGGGATGGTGACTCT23060.11128312434369016No Hit
GCCGCTGAACCTTGATGGGACCCCACTTTGCAAAGTGGACGCAGCATAGA22960.11080054357897337No Hit
CTGATACCAGGCTAAATAACTGCTAATACCCTGACTCGCCCGACAAGGGA22870.11036622089072828No Hit
GCTAATACCCTGACTCGCCCGACAAGGGATGGTGACTCTGTCTCCTGTAG22770.1098836401260115No Hit
GACGCAGCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGCTGATA22520.10867718821421953No Hit
GCACATCTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAGCTGAGC22250.10737422014948422No Hit
CTGCTAATACCCTGACTCGCCCGACAAGGGATGGTGACTCTGTCTCCTGT21940.10587821977886219No Hit
GCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGCTGATACCAGGC21720.10481654209648526No Hit
GCGGTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21670.10457525171412686No Hit
CCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAAGTGG21590.10418918710235345No Hit
CCCCACTTTGCAAAGTGGACGCAGCATAGATCAGGAGCTTAGGGGCTTCC21500.10375486441410835No Hit
CGCCCGACAAGGGATGGTGACTCTGTCTCCTGTAGATGCAGAGAATGAGG21290.1027414448082031No Hit
GTGGACGCAGCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGCTG21220.10240363827290135No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT20930.10100415405522269No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)