FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10598330
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA110218610.39961956270469No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG1868031.7625701407674605No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT1665861.5718136725314271No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1363581.2865989264346365No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG1220481.151577654215334No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG912380.8608714769213641No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC630490.5948956109122853No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC545370.5145810707913417No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT512720.4837743304841423No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT408850.3857683238774411No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT268140.25300212391952315Clontech SMART CDS Primer II A (100% over 26bp)
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT260860.246133117198653No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG258490.24389691583485323No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGACAA206740.19506846833416208No Hit
GAAAAAGTATTTGAGATTAGTTATCACATGAAGGGATAACAAGCTAATTT163020.15381668621377143No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT161790.15265612601230572No Hit
CACGCTGCTCGTATCCGACGGGGACTTCTCACAGGAGACGAGGGGGAAAA147640.13930496597105393No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG140920.13296434438255839No Hit
GTTGAAAAGTGGTCTTGAAAAATACTAAAATGAAGGCCACTCTATCAGAA134260.12668033548681726No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC134200.12662372279406284No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAG117220.1106023307445607No Hit
GTTTCTCCTTAATCACAAAGAGAAAACGAGTTAACCTAAAAAGATTGTGA116340.10977201125082915No Hit
GTTATCACATGAAGGGATAACAAGCTAATTTAAAAAACTTTTTGAATACA109760.10356348594542725No Hit
GTGAACACAGTCATTATGAAAATAATGCTCTGAGGTATCGAAAAAGTATT107210.10115744650336421No Hit
ATTTGAGATTAGTTATCACATGAAGGGATAACAAGCTAATTTAAAAAACT106160.10016672438016178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)