FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143501
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT775472.4668991675205447No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT502981.6000631143428934No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA149370.4751708365927035No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC131050.4168918667434812No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT65750.20916169582894995No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT62830.19987268971761105No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57180.18189909912546554No Hit
TGTTGTTCAGCTGCTGCTTTGTGTATGGTTCCACGGACTGGATCTGGGTC34030.10825509519481623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT129800.0127.2421951
CGTTATC72700.0124.959352
GTATCAA135550.0122.119829
GGTATCA135650.0121.869438
AGTGGTA139750.0116.939475
TGGTATC138700.0116.20617
CAGTGGT141750.0115.238384
GTGGTAT141850.0114.1823656
TATCCAC79500.0113.908035
ATCCACC79900.0112.880476
AGCAGTG147050.0112.3157962
GCAGTGG150300.0110.467753
CTCGTAT23700.0104.018448
TCGTATC23750.0103.1888669
CTACACG21100.0102.0530851
ACGACGC21650.098.122535
TACACGA22350.096.021012
TTATCCA95150.095.0965654
CACGACG22850.093.28684
GTTATCC99250.091.095093
Produced by FastQC (version 0.11.5)