FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338951
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA128710.38548034996620195No Hit
TGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC113380.3395677265105118No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG78680.23564287106938678No Hit
CACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTT52330.15672586989147191No Hit
TTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGC51410.1539705134936092No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG50190.15031667131383478No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACC49560.14842985117182012No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT48900.1464531824516143No Hit
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG39690.11886966894692375No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC39000.11680315164852674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAC41600.0135.586062
CGCTATC27600.0133.176531
AGTATTC60250.0132.360357
GAACGGA43700.0132.05671
TCAGTAT60750.0131.39035
GGTCGGT67750.0131.083836
AGGTCGG68000.0130.388675
CGTCAGT62850.0130.11663
TCGTCGA25950.0129.90866
CGGTTCG69000.0129.238339
CTCGAGG70750.0128.908921
CGTCGAG26450.0128.001057
TACACGA20900.0127.65281
GTCGAGT26800.0127.140938
GTCAGTA64350.0126.968994
TCGTTAC26800.0126.8704158
CTCGTCA66700.0126.519051
TGCGTAA16200.0125.7516255
TCGGTTC71050.0125.505678
GCTAGCC32600.0125.4286659
Produced by FastQC (version 0.11.5)