FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338951
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1291523.868041190182186No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT636591.9065568796906573Clontech SMART CDS Primer II A (100% over 26bp)
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA284170.8510756821528678No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT232130.6952183485172438No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT210170.6294491892813042No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC139690.41836492958417176No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT98760.29578151940534614No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT96410.2887433807803709No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG55330.16571072771058934No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC54220.1623863303175159No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG47870.14336838126705065No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT47540.14238004690694772No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC42760.12806417344848725No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40670.12180472250116878Clontech SMART CDS Primer II A (100% over 25bp)
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT39740.11901941657724237No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA38830.11629400970544343No Hit
GCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTC33870.10143904477783591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)