FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238458
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA581542.5979491239058317No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC243351.087132302683365No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG158850.7096402970258991No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT144820.6469632220037187No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG140620.6282003057461878No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT132800.5932655426190708No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT126520.5652105154530485Clontech SMART CDS Primer II A (100% over 26bp)
GCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTC117080.5230386274837411No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG99180.44307286533855006No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG86650.3870968318369163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83240.3718631307802067No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG57470.25673923745721383No Hit
TAGCTGCTGGCCGCGGTACTGTTGGTCATGGAGCTGTTGATATCTTCCTC36570.16337139227092937No Hit
CTGCTGGCCGCGGTACTGTTGGTCATGGAGCTGTTGATATCTTCCTCCAG26710.11932321267586884No Hit
GTCCCAGGTCACCATCACGCCCTGGTGCCTGGGGCGCCCCACGATGGAGG26590.11878712935422509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)