FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3132647
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAC794600.0142.427927
TGTCCAA994650.0142.316737
CTGTCCA994100.0142.286076
AGAGACT796650.0142.13428
TGCTAGA796750.0142.052674
GCTGCTA795550.0142.05052
GAGACTT795950.0142.015569
TCTCTGT995500.0141.993563
CTGCTAG796500.0141.9633
CTAGAGA797500.0141.928186
TTCTCTG997250.0141.81712
TCCAAGT1004350.0141.54369
CTAGTGC436900.0141.473886
GCTAGAG799850.0141.45685
ACTAGTG437100.0141.44235
TACTAGT437800.0141.31554
CTCTGTC1003150.0141.139744
TTACTAG438300.0141.106933
GTCCAAG1008600.0141.095878
TAGTGCC439250.0140.865527
Produced by FastQC (version 0.11.5)