FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3132647
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT2419957.724936770724566No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT982743.137091411831592No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG259400.8280537194264147No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT228140.728265904201782Clontech SMART CDS Primer II A (100% over 26bp)
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT161890.5167834103236018No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA83340.26603699682728377No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC74990.23938222212716592No Hit
GCCTGGTGGAGGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTC60620.19351047213426856No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA60580.19338278459079494No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA59460.18980753337353362No Hit
GGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTATGTTTGTG52800.16854755738517618No Hit
GCTGGTACCAGGCTATGTTTGTGCGAACACTCTGACTGGCCCTGCAGGAG50500.16120552363544313No Hit
GGAAATTCTCACTGAGGCCCCAGGCTCCCTCGCCTCAGACCCAGATTGGA48240.1539911774291837No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC47090.15032016055431716No Hit
GGTTTCTGCTGGTACCAGGCTATGTTTGTGCGAACACTCTGACTGGCCCT46710.1491071288913178No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTATGTTTGTGCGA46490.14840484740221288No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC45650.1457234089892669No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT44440.14186086079918994No Hit
GCCTGGCCAGGTTTCTGCTGGTACCAGGCTATGTTTGTGCGAACACTCTG41530.13257159201148422No Hit
GCCAGAAAATCTGGCTGGGACACCGGTGGGCCTGGTGGAGGCACCATAGA39330.12554877712043522No Hit
GCGAACACTCTGACTGGCCCTGCAGGAGAGGGTGCCTCTTTCCCCTGGAG39080.1247507299737251No Hit
CTCTGACTGGCCCTGCAGGAGAGGGTGCCTCTTTCCCCTGGAGACACAGA38730.12363346396833093No Hit
GTTTGTGCGAACACTCTGACTGGCCCTGCAGGAGAGGGTGCCTCTTTCCC38460.12277157304988401No Hit
GGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACC38090.12159046327275304No Hit
GGAGAGGGTGCCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGGAGACT36530.11661064907728191No Hit
AGAAAATCTGGCTGGGACACCGGTGGGCCTGGTGGAGGCACCATAGATGA36250.11571683627296661No Hit
GCCTGGCCAGGTTTGTGCTGATACCAGACTAAGAAGTTCTTAACACTCTG36010.11495071101212488No Hit
GGTGCCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGGAGACTGCGTCA35040.11185428808288965No Hit
CCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGGAGACTGCGTCATCAC35000.11172660053941602No Hit
GGCAAATCGTCCTGTGAAGTCCTGGGCATATGTTGGGGTGCCAGTCTTGG34060.10872594326778601No Hit
GCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCCGTCCC33530.10703408331676056No Hit
GTGAGTGAAGGTGTATCCAGAAGACTTACAGGAAATTCTCACTGAGGCCC33460.10681063011568172No Hit
GTTTCTGCTGGTACCAGGCTATGTTTGTGCGAACACTCTGACTGGCCCTG33350.10645948937112928No Hit
GGATAGTGAGTGAAGGTGTATCCAGAAGACTTACAGGAAATTCTCACTGA32840.10483147319184064No Hit
GGCCTGGTGGAGGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTT32550.10390573850165691No Hit
GTGAACTCCGTCCCAGACCCAGTGCCAGAAAATCTGGCTGGGACACCGGT32330.103203457012552No Hit
GGTGTATCCAGAAGACTTACAGGAAATTCTCACTGAGGCCCCAGGCTCCC31760.10138390951805294No Hit
GGTACCAGGCTATGTTTGTGCGAACACTCTGACTGGCCCTGCAGGAGAGG31680.10112853443110571No Hit
GGCCCTGCAGGAGAGGGTGCCTCTTTCCCCTGGAGACACAGACAGGGTGG31590.10084123745829007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)