FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15922889
Sequences flagged as poor quality0
Sequence length105
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT607920.38179001310628996No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA310680.19511534621638071No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC248740.15621537021328227No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG233630.1467258862383579No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT229250.14397512913642746No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG207260.13016482122057121No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA199000.12497732038451062No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC185350.11640475544356303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG34750.058.9782685
ACCGCGT35150.058.1589666
CGCGTTC39150.052.343238
CCGCGTT39300.052.0174947
AGACCGC41600.049.5047074
CAGACCG49750.042.290453
GAGTCTT238850.026.1565594
AGTCTTG256100.025.2066145
GTCTTGG259300.024.5488056
TACGGAG49850.022.68887334-35
TGGAGTC293800.022.561882
GGAGTCT291200.022.542323
ATACGGA47900.022.37248832-33
GAACGAT48050.021.78757544-45
AATACGG52150.021.593332-33
GCGTTCT96350.021.5769219
GCAACGC49650.021.3363780-81
TTGGAAG294600.021.321659
CTTGGAA298450.021.2953788
TCTTGGA305850.021.2818417
Produced by FastQC (version 0.11.5)