FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7121740
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT178070.2500372100076667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG5450.033.9887055
ACCGCGT5800.031.1187486
CCGCGTT6400.028.9424927
CGCGTTC6700.027.6465598
AGACCGC8200.022.0109774
CTCGCTA11700.019.4933551
CCCGTCT23350.019.3316061
GTCTTGG95200.019.3081446
TGGAGTC93450.019.1115722
GAGTCTT95400.018.8695014
GGAGTCT95200.018.6604673
CAGACCG11850.018.4385763
AGTCTTG100500.018.2899045
CGTACGC3156.828486E-918.0941095
CCGTACG3351.4617399E-817.0139834
TCGCTAT12350.016.5382422
TCTTGGA114450.016.3920237
CCTACGT55950.016.3001124-25
CTTGGAA112850.016.1193858
CCCTACG54800.016.07876624-25
Produced by FastQC (version 0.11.5)