FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7121740
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG3550.028.0822859
ATCGGGA14200.024.4065592
GCGACAT14400.019.4501027
GGCGCGT7100.017.383544
CGCGTGC10500.016.2786661
GGCGATC7800.015.8252572
TGCGACA17550.015.6884556
CGCCGGA8950.015.3894346-47
CGACATC19150.014.8736618
ATATCGC7400.014.7547388
GGGCGAT9450.014.5704111
TCGGGGG16350.014.52465938-39
GGCGCCG11850.014.42869944-45
GCCGGAT11300.014.29052146-47
GCTGCGA20500.013.8937854
CTGCGAC20000.013.766915
GAGCGGC17450.012.7862729
GGGGCGC12450.012.77945642-43
AGAGCGG16700.012.5075328
CCGGATC12500.012.15901148-49
Produced by FastQC (version 0.11.5)