FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7084126
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT315440.44527722968225014No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA103190.14566369937519463No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC96520.1362482824275006No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG93770.1323663638958426No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC71330.10068990867751365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG17900.057.84235
ACCGCGT18200.056.6278956
CGCGTTC18200.056.6266988
CCGCGTT18650.055.260377
AGACCGC19200.053.6789054
CAGACCG22950.046.150513
GCGTTCT25400.040.762029
GAGTCTT108550.027.389854
CCGTACG9450.027.1398284
TGGAGTC110950.027.0547872
GGAGTCT110550.026.9378643
CGTACGC9650.026.5771565
GTCTTGG113800.026.3347476
AGTCTTG117450.025.6780935
AGGCGTA12200.023.746688
TCTTGGA128300.023.6911537
TTGGAAG127150.023.3824969
CTTGGAA128850.023.2582918
GAACGAT23100.023.0339144-45
TACGGAG24150.022.81850234-35
Produced by FastQC (version 0.11.5)