FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7084126
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA12650.029.2755952
GGCGATC12850.026.2344651
GGCGCGT22350.023.7895853
TGGCGCG22950.023.3774622
TGCGACA28500.023.1531546
GCGACAT29500.022.3683037
GCGCGTG25300.021.0157014
CTGCGAC31450.020.9822865
GGGCGAT18200.019.8270991
CGCGTGC28150.018.8885365
CGACATC36250.018.7270038
CGATCTG17900.018.5648483
TCACCCG16550.018.0712813
AGAGCGG16500.016.9749438
TCAGACG9600.016.8154812
GCTGCGA40050.016.3577274
GGCTGCG41650.016.2992423
GTGGCGC33800.016.2951471
GGTGGCG26150.016.1597731
CGGCCCG20850.015.7102588
Produced by FastQC (version 0.11.5)