FastQCFastQC Report
Thu 2 May 2024
EGAF00005068456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46020250
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG3766090.8183549633041977No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3533090.7677250775473841No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1178930.25617635714712544No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA946400.20564860034441362No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC859160.1866917280979569No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTC810440.17610508417490126No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT803600.1746187819492506No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT792790.1722698160049109No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT723730.15726337862136777No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC720780.15662235646264416No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG662970.14406049510813174No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC617360.13414964064732374No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA603680.1311770361960224No Hit
CAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAA600260.1304338850831971No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG590320.12827396635176908No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA589790.12815879965884583No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC584430.1269940949907921No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG560220.12173336737631804No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA552170.11998413741776719No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC549590.1194235146484428No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT528590.11486030606091885No Hit
CTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCT525640.11421928390219524No Hit
CCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCT525140.11411063607868276No Hit
CGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA513240.11152481787908583No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT507680.11031665408162711No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC494940.10754830753852923No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG494400.10743096788913577No Hit
CCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGT493670.10727234206680755No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA485350.10546444228355996No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA480810.10447792004606668No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG478440.10396292936261754No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT469610.10204420879938722No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG469170.10194859871469626No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG466670.10140535959713387No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC464550.10094469282544097No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG463460.10070784057018378No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT462300.10045577761963484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)