FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4615974
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT88910.19261373655917471No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA82480.17868384873918267No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC52850.11449371248624883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT17950.057.424566
CGCGTTC18300.056.06558
CCGCGTT18700.054.8662347
GACCGCG18850.054.4308135
AGACCGC19650.052.456544
CAGACCG21450.048.4974863
GCGTTCT23100.044.415529
CCGTACG3200.026.7192924
TACGGAG22050.025.85030434-35
TCAGACC41750.025.8268722
AATACGG22450.025.70709232-33
CGTACGC3200.025.2348885
CGCGACG1901.19271135E-825.0005051
CTCAGAC49550.023.4868551
AACGATC25150.022.56955146-47
CGGAGAG25450.022.11686536-37
GTACGCC4300.022.0934726
ATACGGA21850.022.06518732-33
AGAACGA25950.021.6907244-45
TGACGGC26900.021.45443262-63
Produced by FastQC (version 0.11.5)