FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4615974
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47810.10357510679219596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT8450.024.1723754
GGCGATC6100.024.1382852
ACGTGCG5150.021.2225768
GCGCGTG10500.019.4549085
TGGCGCG11300.019.3356443
GCGTACG1403.573041E-818.62529294-95
CGCGTGC11200.018.2415526
CGTACGG1254.650621E-617.06754394-95
GCGCGCG3903.0013325E-1017.0559066
CGATCTG9300.016.855384
CGCGCGC3953.601599E-1016.841297
CGTGCGC6800.016.7728989
TAACACG7750.015.3230734
TATCGCG1258.65696E-515.21321822-23
CGCACGA4300.014.9267328-29
GGGCGAT10400.014.6142661
GGTGGCG16050.014.5004481
TCGCACG4350.014.20851726-27
CGCGAGT4350.013.65876912-13
TGCGACA10850.013.5751096
Produced by FastQC (version 0.11.5)