FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6729938
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA179690.26700097385741145No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG102610.15246797221608877No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC82220.12217051628113068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT6150.031.6673286
CGCGTTC6300.030.158698
CGGCATG33050.029.0323773
CCGCGTT6450.028.7208887
CGTACGC18500.025.419455
CCGTACG18700.025.1475834
GTACGCC20800.022.8370136
TCCGTAC21150.022.683693
GACCGCG8650.022.5149215
AGACCGC8850.020.932644
TACGCCA23000.020.6521457
GTATTAG49650.019.6122599
TTCGCGC3701.4188117E-1017.9733496
GCATGTA54650.017.6444665
TTCCGTA28800.017.318072
GTCGGCA70450.017.2608281
GTCGGTT7350.016.8030721
GTCCGTT6850.016.6426831
CCCGTCG14100.016.5074371
GTCGTAT3854.660251E-916.0392951
Produced by FastQC (version 0.11.5)