FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8359299
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT333670.399160264515003No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC222320.2659553151526222No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG210930.25232977071402757No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA184780.22104724331549808No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA135020.16152072081642252No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA125430.15004846698269794No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA112290.13432944556714624No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC106020.12682881662684872No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG102550.12267775084968251No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA100140.11979473398427308No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC87130.10423122800129532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT23400.056.4321866
CGCGTTC24200.054.5660068
CCGCGTT24600.053.4856647
GACCGCG25750.051.835475
AGACCGC28450.046.7491464
GCGTTCT37200.035.8803029
CAGACCG38800.035.380513
AATACGG30850.025.867232-33
TACGGAG32900.024.47198134-35
ATACGGA29650.022.82892632-33
GTCTCGC56750.022.4319424
CAACGCC34250.022.12039482-83
CAATACG31950.021.11119530-31
AACGATC37150.021.09686346-47
CTCGCTA59050.020.9951296
AGAACGA37650.020.87977244-45
GGAGTCT164000.020.738013
GGGGTCT76600.020.7118191
TGGAGTC166200.020.377762
TCGCTAT61150.020.2738747
Produced by FastQC (version 0.11.5)