FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8359299
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA194240.23236398171664874No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN184570.22079602607826326No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA167440.2003038771552495No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG128550.1537808373644728No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC115330.1379661141442602No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA104780.12534543865460487No Hit
GGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCT91100.10898043005759216No Hit
GGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTG90780.10859762283894857No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG87990.10526002240139994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC14850.035.19192
TGGCGCG23800.034.3355183
GGGCGAT15550.032.995661
GGCGCGT25500.032.607394
GCGATCT20050.026.5396863
GGTGGCG31000.026.512331
GCGCGTG33000.025.1969225
GTGGCGC33150.025.08022
CGCGTGC36450.022.680456
CGATCTG22800.022.2980614
GCGTGCC42800.019.426957
GCGCGCG8150.016.905986
CGTGCCT52250.016.0959118
GCATTCG9450.015.0827863
TGCGACA30150.014.8129136
GCGACAT31400.014.3748837
CGCGCGC8100.014.0778587
GTTGTCG7950.014.05482326-27
TCAGACG14050.013.8638542
CGTGCGT11850.013.6329298
Produced by FastQC (version 0.11.5)