FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8070667
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT335800.41607465653086667No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG206680.2560878797254304No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC150820.18687426949965846No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC95460.11828018675532023No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT86300.10693044329545501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT8900.042.69316
CGCGTTC10150.037.90218
CCGCGTT10400.036.534547
AGACCGC13450.029.3098494
GACCGCG14000.027.1406155
TGGAGTC142350.024.9575462
GAGTCTT142450.024.8066564
GGAGTCT142650.024.8051743
AGTCTTG150200.023.6215525
TTGGAAG164250.021.426799
GTTTGAC50050.021.2569855
GTCTTGG168600.021.0718066
CTTGGAA169950.021.0714538
CTCGCTA26050.020.238256
TCTTGGA183650.020.0944947
TCGCTAT26850.019.9885447
GCGTTCT19850.019.8592059
GTCCGAT5800.019.6613451
CGCACTA77150.018.28423982-83
CGCTATG29100.018.279718
Produced by FastQC (version 0.11.5)