FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8070667
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT14650.033.7044561
GGCGATC15850.030.851052
GGTGGCG36650.023.706571
GGCGCGT37450.022.942333
CGATCTG25000.021.0762714
TGGCGCG43500.019.8629672
GCGATCT26400.019.5989743
TGCGACA31350.017.2630886
GCGACAT32300.016.9023277
GCGCGTG52700.016.4835684
GTGGCGC53350.016.4637571
CGCGTGC53600.016.1193315
CTGCGAC37100.015.0990115
GCGTGCC58550.014.8386
TATACGC5501.2732926E-1114.6736267
GTCGACG3002.582193E-514.2433741
ATCCTAG39500.013.5810246
CGTGCCT69350.013.2802567
CGGTGGC36500.013.2677991
ATATACG9100.013.0421696
Produced by FastQC (version 0.11.5)