FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8901509
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT196740.22101870592952272No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA169160.19003519515623699No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG168390.18917017328185592No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC116800.13121370769832397No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC99750.11205965190845732No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC94010.1056113070267075No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG93320.10483615755485952No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA90370.1015221127114515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATG32300.028.6770633
CGTACGC23650.028.5206745
CCGTACG23950.028.3617574
ACCGCGT8050.027.7334926
CGCGTTC8550.026.1110658
CCGCGTT8650.025.8092027
GTACGCC27150.025.3688496
TCCGTAC27250.024.9271223
TACGCCA30550.022.3894847
CTCGCTA29600.022.3062041
GACCGCG10050.022.2143925
TCGGGAT16700.022.1860872
GTTCGAT60050.021.5949521
TCGCTAT31600.021.3453792
CGATTAG62600.019.6529624
CGCTATG34600.019.631913
TTCCGTA35150.019.5952
GAGTCTT106200.019.5460724
TTCGATT66950.019.1564832
TCGATTA63800.019.1344093
Produced by FastQC (version 0.11.5)