FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8901509
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN195980.22016491810545832No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA98880.11108228953090989No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT15600.025.5825864
ACGTGCG16950.023.8260028
GGCGATC10800.021.994372
GTCTAAC21900.021.9088951
CGTGCGC19650.020.7948139
TAACACG19700.020.7406314
TCAGACG19100.020.1473052
GCGACAT22350.019.556137
TAGCATA42100.019.4117859
TCGTGCG15550.018.6368317
TGCGACA23250.018.5944676
CGCGTGC21250.018.5560026
GCGCGTG21800.018.0893765
CAGACGT21750.017.6932723
AACACGT23700.017.4410615
GTAGCAT46600.017.3326518
CCAGTAG45350.017.0770285
GCCAGTA46900.016.917394
TGGCGCG25050.016.5003783
CAGTAGC47950.016.3478496
Produced by FastQC (version 0.11.5)