FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7942685
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG149090.18770730552703524No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA120530.15174969169745495No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG95200.11985871276526767No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC95060.11968244995237756No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT90440.11386577712700428No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT84300.10613539376168135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT9150.049.3136526
GACCGCG9500.047.9967965
CCGCGTT9750.046.276347
CGCGTTC10750.041.971578
AGACCGC11300.040.3512884
CAGACCG17750.026.491693
GCGTTCT17300.026.0805979
TATCGCG2800.022.05258422-23
GTCGGTT6850.021.5045281
CTCGCTA36500.017.6974496
TACGGAG14300.017.44028334-35
ATACGGA14250.017.00127432-33
CGCACTA67400.016.55984382-83
TCGCTAT38950.016.4613847
AATACGG16500.016.4103232-33
CGATCGG74700.015.99234370-71
CAATACG14700.015.8344230-31
CGCGACG2457.3162926E-515.5161041
TCGTGCG6450.015.4664234-35
ATCGGCA79200.015.20363872-73
Produced by FastQC (version 0.11.5)