FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7942685
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG81090.10209393926612978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG24400.026.4605672
GGCGCGT26600.023.9130483
TATCGCG3450.019.95735722-23
GCGCGTG33050.019.3898334
CGCGTGC34150.018.765985
GTGGCGC39600.016.4254231
GCCGGTA14400.016.1523026
CCGGTAG14700.015.8228617
GGTGGCG32150.015.8013421
TTGCGCG2751.1365764E-515.5351738
GCGTGCC42250.015.3919866
ACGTCGG5255.456968E-1215.3705866
CGGTAGC15950.014.2852178
CGCGGGT4101.75487E-713.895961
GGCCGGT17150.013.5631375
CGATCGA2450.001294815913.5626244
TCCGCGG5151.5412297E-812.9040817
CGGTGGC29400.012.1116991
GCGACAT10200.012.0998367
CGTGCCT56750.011.7939587
Produced by FastQC (version 0.11.5)