FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8152927
Sequences flagged as poor quality0
Sequence length105
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG451680.554009621329861No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT299350.36716874810727484No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC257450.31577616235249006No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA217510.2667876211819387No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT185490.227513382617065No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT177800.21808118728402695No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG174050.2134816121866417No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA147980.1815053661096193No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT116790.14324916683296687No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC107750.1321611244648701No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG98730.12109761316395941No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG94160.11549226431194588No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT93100.11419211775108497No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA92040.11289197119022407No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC91330.11202111830511913No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC88870.10900379704123439No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG85750.10517695056020986No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT84580.10374188312982564No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG84350.10345977585718602No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC82410.1010802623401387No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT82390.10105573127295266No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT12900.060.242116
CCGCGTT15000.051.8082167
CGCGTTC15150.051.2952658
AGACCGC16550.047.560094
GCGTTCT22450.034.8362169
GACCGCG25900.030.5818485
GAGTCTT112650.027.8173664
TGGAGTC115000.027.4211142
GGAGTCT119050.026.4051023
AGTCTTG125100.025.6821215
GTCGTAA15050.025.65580486-87
GTTTGAC75450.025.5887375
TCGTAAC15350.025.31509288-89
CGCGTAT1050.001727291523.5749531
AGTCGTA17750.022.58989586-87
TTGGAAG141050.022.3189759
TACGGAG20250.021.5103734-35
CGTAACA18200.021.48690888-89
TTGACTG91850.021.4483647
ATACGGA19200.021.39770132-33
Produced by FastQC (version 0.11.5)