FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8152927
Sequences flagged as poor quality0
Sequence length105
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG138570.16996349899858051No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT134170.16456666421764846No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC129890.15931701583983274No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG103640.12711999015813583No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG89050.10922457664590889No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT86530.10613366218046598No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG86060.1055571821015937No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA84000.10303048218143006No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC83280.10214736376273209No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT16650.042.843181
GGCGATC19900.034.600672
GGTGGCG46500.034.51641
GGCGCGT67350.028.5382631
TGGCGCG61650.025.9518433
CGATCTG27450.025.6234024
GCGATCT28750.024.9820983
GTGGCGC73000.021.9859332
TATCGCG4450.021.14049122-23
GCGTGCC95800.020.0612034
CGGTGGC43650.019.7468781
TATACGC10300.019.714187
GCGCGTG102650.019.0134092
CGCGTGC109150.017.8347723
CGGCTAT12700.017.552631
TGCGACA39000.017.26854712-13
CGTGCCT111650.017.2566975
ATATACG12200.017.0498896
AACCTTG61700.017.018355
GCGACAT40500.016.6309097
Produced by FastQC (version 0.11.5)