FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11058704
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT287930.2603650482009465No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC180120.16287622853455522No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG154240.13947384793010104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT11000.041.4552426
GACCGCG11500.039.652845
CCGCGTT12150.037.530827
CGCGTTC12450.037.0079848
AGACCGC16050.028.707654
CCGTACG4950.024.9499174
CGTACGC5250.023.5242065
GGAGTCT156250.019.5779293
CAGACCG24150.019.4723783
TGGAGTC157600.019.4403672
GTCCGAT8600.018.2272171
GAGTCTT166850.018.2202664
GCGTTCT25600.017.9980249
GTCTTGG175500.017.8364836
CGATCGG75200.017.65456670-71
AGTCTTG180250.017.577275
CTCGCTA30100.017.5170651
CGCACTA75750.017.24420482-83
GTCTGGA125150.017.1179031
ATAGCGC80250.017.01711378-79
Produced by FastQC (version 0.11.5)