FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9176535
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT229560.2501597825322957No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG150350.16384179867455417No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA128490.14002017101226116No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC114650.12493822559386522No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC101190.1102703798329108No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA101090.11016140623884724No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA93310.1016832606207027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT18600.050.8209086
CGCGTTC20800.045.2162638
GACCGCG21300.045.0478485
CCGCGTT21250.044.4822737
AGACCGC23350.041.0928964
CAGACCG33700.028.7542843
GCGTTCT33450.028.542559
CGTACGC13350.022.771995
TACGGAG26800.021.97757134-35
CCGTACG14000.021.714724
AATACGG27100.021.64663732-33
CAACGCC28200.020.0442982-83
ATACGGA25100.019.58661732-33
CTCGCTA42850.019.28866
GTACGCC16450.019.0581326
CGGCATG22550.018.958253
TCCGTAC16600.018.885923
GCAACGC25700.018.85210680-81
GTCTCGC44950.018.8101654
GGAGTCT128750.018.6314413
Produced by FastQC (version 0.11.5)