FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9176535
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN200000.21794718812710895No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA155150.1690725311896048No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA95190.10373196418909751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT17350.028.4784744
GGCGATC12900.024.306522
TGGCGCG24400.021.0285643
CGCGTGC23850.020.9144156
GCGCGTG23900.020.6743815
TCACCCG22600.020.1808053
CGGCCCG22750.019.839788
CGATCTG17850.018.6312774
GGTGGCG28150.018.394811
CACCCGG28300.016.7878994
ACCCGGC28550.016.6414415
GCGTGCC32700.015.3998757
TCTCGCG13150.015.173397
CTCGATT18250.014.97455948-49
TAGCATA36850.014.8274659
GGGCGAT21950.014.7170781
GCGATCT21400.014.6523023
TGCGACA23350.014.4449596
GCGACAT24000.014.0542017
GAGTGCG29950.013.97023128-29
Produced by FastQC (version 0.11.5)