FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3969539
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT41580.10474767976835597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC7000.029.8433571
GGCGCGT17200.026.4993881
TGGCGCG15550.024.7300952
TGCGACA15650.020.0204286
GCGACAT15550.019.8441377
GCGCGTG23700.018.8300152
GGGCGAT7700.018.4979481
TATCGTA3105.631591E-918.3767535
CGATCTG11600.018.0064243
TCGTAGC3501.3242243E-917.6329337
CTGCGAC17350.017.2381365
CGCGTGC26500.016.8389423
GTGGCGC22900.016.5862261
GCGATCT12800.016.3197632
GGTGGCG18300.016.0854661
GCTGCGA20100.015.3514754
GCGTGCC29750.015.1589624
CGGTGGC16950.014.845651
TATCGCG1858.354709E-714.11732722-23
CGACCCG4051.5119076E-714.0661575
Produced by FastQC (version 0.11.5)