FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6302945
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG74960.1189285326145159No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT20400.025.1310463
GGGCGAT10100.024.9134331
GGCGATC10050.024.5624432
TGGCGCG22750.022.7446542
GGTGGCG23000.022.2933331
CGCGTGC26850.019.2718475
GCGCGTG27350.018.9186274
CGATCTG15950.017.2618664
GTGGCGC31350.016.6584361
TATCGCG2800.016.11182422-23
GCGATCT17100.015.8232523
GCGTGCC33800.015.3087816
GCGACAT19050.014.2041137
GAGTGCG18800.013.26260728-29
TGCGACA19350.013.2477956
GCCACGA19550.013.23793518-19
GCGTCCT19500.012.78656332-33
CGTGCCT40300.012.7220298
GCTGCGA22400.012.5032944
CACGAGC20100.012.28531820-21
Produced by FastQC (version 0.11.5)