FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109616
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG81810.19906969410280667No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT77460.18848476353995117No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC72150.17556384830115515No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG69560.16926155631085726No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT64260.15636497424576895No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC61970.1507926774667025No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG60100.14624237398336No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT58270.1417894031948484No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA57780.1405970776831704No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG54750.13322412604973313No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC54660.1330051274863637No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG53650.1305474769418846No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA46490.1131249245671615No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC45080.10969394707437385No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG43650.10621430323417079No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG42990.10460831376946167No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC42700.10390265173193798No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA41890.10193166466161314No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT41290.10047167423915032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT10800.049.677491
GGCGATC15350.035.2615551
GGCGCGT45650.026.7299021
TGGCGCG44250.026.7159062
GGTGGCG24450.026.0214181
CGATCTG21850.024.7691353
GTGGCGC49850.023.6206321
GCGATCT23100.023.4288123
GCGCGTG53900.022.6374862
CGCGTGC57850.021.1725793
ATCGGGA9350.020.8188192
GCGTGCC67050.018.3380474
TATCGCG4650.017.87228222-23
CGTGGCG21600.017.8047641
CGGTGGC20400.017.4556541
TATCCTA21450.016.82135
GTATCCT23000.016.7189624
ATCCTAG23050.016.6828986
CGTGCCT74150.016.5829525
TGCGACA34900.016.187476
Produced by FastQC (version 0.11.5)