FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10685920
Sequences flagged as poor quality0
Sequence length105
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG261040.24428406725859825No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC119670.1119884857831614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC18900.027.2597482
GGGCGAT20100.025.879371
GGCGCGT35100.023.852841
TGGCGCG41000.020.2981013
TATCGCG4950.020.00610722-23
GGTGGCG45450.018.5300051
CGATCTG29750.018.3153554
GCGCGTG48700.017.1912732
CGCGTGC48950.017.0014743
GTGGCGC56400.015.37161351
GCGATCT35750.014.9650343
GCGACAT36300.013.7799337
GCGTGCC63300.013.6944314
TGCGACA37550.013.3212146
TGCGCTA195550.012.4133382
GTGCGCT200300.012.1934461
GCGCTAT204000.011.8986013
TATGCCG206850.011.8038517
GCCACGA36500.011.7354218-19
TGCCGAT208800.011.668429
Produced by FastQC (version 0.11.5)