FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4704085
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT120640.25645795090862517No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA87770.18658251285850488No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC63490.13496779926383132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT4550.046.9780246
GACCGCG5200.042.0192345
CGCGTTC5450.039.2201848
CCGCGTT5900.036.2288137
AGACCGC6750.032.3703734
CGTATCG1405.812526E-727.1428553
CAGACCG9000.024.2777773
CGTACGC6600.020.8712125
CTACGCG1201.4158832E-719.79166886-87
CCGTACG7000.019.678574
GCGTTCT11450.019.082979
CGCGACG2001.2861152E-519.01
GTACGCC8000.019.06
TCCGTAC8300.018.8855443
CCCGTCT17450.018.2378221
GTCGAAT4303.6379788E-1217.674421
GTCTTGG73950.017.4070326
GCCGGAC6300.017.341271
GAGTCTT74600.016.8096524
TGGAGTC74450.016.7159182
Produced by FastQC (version 0.11.5)