FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4704085
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48580.10327194342789299No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGCG7050.019.5446937
ATCGGGA8000.017.8112282
ACGTGCG3501.3078534E-917.6485568
CTCGCGC8200.016.8042078
GGCGATC5153.6379788E-1215.6784922
CGATCGA3806.88633E-815.0000484
ATCTCGC9600.014.3522156
ATATCCG13200.014.0341344
TGCGACA8500.013.4148096
TATCCGC13600.013.2737985
GTTAGCG2550.001737384113.04342658
CGCCGGA4600.012.91411846-47
GCGACAT8200.012.7476117
TCGCACG3059.513315E-1012.47015326-27
GCGACCC9600.012.3714125
CGTGCGC5001.4381658E-712.3543849
AATCTCG13550.012.2709815
CTGCGAC9350.012.1941125
CGCACGA2551.848548E-712.1187328-29
CGCGAGT2804.8767106E-811.88105212-13
Produced by FastQC (version 0.11.5)