FastQCFastQC Report
Fri 10 May 2024
EGAF00005068551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10589321
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length105
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT349950.33047444685074706No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG283010.26725981769747087No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC250450.23651185944783426TruSeq Adapter, Index 4 (100% over 50bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA224870.2123554475305829No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA193590.182816254224421No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG175890.1661013014904355No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC172850.1632304847496832No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT137580.12992334447128384No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC124470.11754294727678952No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC119380.11273621793125357No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA115460.10903437529186243No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA115220.10880773186496093No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG107590.10160235958471747No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)