FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10589321
Sequences flagged as poor quality0
Sequence length105
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG204570.19318519100516454No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT122150.1153520608167417No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC116210.10974263600092961No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG105900.10000641212028609No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA31600.039.3461882
GGCGATC27000.033.574971
GGGCGAT23850.030.5321431
GGCGCGT78300.024.7367881
GGTGGCG67300.022.5970461
TGGCGCG87850.021.7651442
TATCGCG7900.021.62285622-23
CGATCTG43700.021.536133
GCGATCT44250.020.5977882
GCGCGTG96700.020.1830022
CGCGTGC97900.019.8334773
GCCGGAT25400.018.51264246-47
GTGGCGC110050.017.7801481
AGGGGCG34300.017.1800810-11
GCGTGCC116000.016.865924
CGGGAGA69450.016.6170754
TGCGACA57750.016.379826
TCGGGGG53650.015.31589938-39
GCGACAT64350.015.1616147
CGTGCCT130350.015.0844615
Produced by FastQC (version 0.11.5)