FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8591458
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT138790.1615441756218793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT6150.030.1222256
CGTACGC5400.024.6298545
CGCGTTC7700.024.0583828
GACCGCG7900.023.449585
CCGTACG5750.023.1306464
CCGCGTT8200.022.5914067
GTACGCC7600.019.3751756
AGACCGC10400.017.8126644
TATCGCG2053.3352445E-416.2194719
CCCGTAT8800.015.6536431
GGAGTCT98650.015.2636973
CCCGTCT29300.015.2391351
GTCGGTT10000.015.2002291
GAGTCTT101750.014.9853954
GTCTTGG107900.014.8356316
TGGAGTC104650.014.6155182
CGTATAC42600.014.43951792-93
TTCGCGC2651.4323926E-414.3395867
CCCTACG50100.014.31653924-25
GTCCGTT8300.014.3074441
Produced by FastQC (version 0.11.5)