FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24506086
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG778360.31761906001635676No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT616540.25158648345557916No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC567020.23137925819733104No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT516210.210645633088858No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT511270.20862980730582598No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG421700.17207970297664016No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA415450.1695293161053952No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA368760.15047690602244684No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC358630.146343238981533No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA312640.12757647222816404No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT245070.10000372968576052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG229950.038.90633870
CCCGATC266000.025.11090569
GACCGCG36850.024.128815
CCGCGTT38400.023.426167
CGCGTTC39050.023.3030558
ACCGCGT38700.023.154916
TATGCCG19050.022.99764848
ACCCGAT277400.022.10755268
CACCCGA284750.021.24528367
TCGTATG21500.021.0172245
GATCGGA22150.020.5523511
AGACCGC44000.020.2868184
CCTACGT233950.019.27015125
CCCTACG235250.019.2227224
ATACGGA48250.019.22067633
GACTACC238700.018.93076119
AATACGG48150.018.82738932
TACCCTA243400.018.70772422
CTACGTT242750.018.68604726
ACCCTAC242950.018.6849623
Produced by FastQC (version 0.11.5)