FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17008433
Sequences flagged as poor quality0
Sequence length105
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA787150.4627998358226181No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT596340.3506143099720004No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG574320.3376677910304847No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG526500.30955232619019046No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT514910.3027380594085299No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC386380.22716966342519618No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC354630.20850245287146676No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA306550.1802341226849058No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC215610.12676652810990877No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG211480.12433832087882524No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC208620.12265680206989087No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC205360.12074010580516148No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA201870.11868818250334995No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA201380.11840009012000106No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT196180.11534278319466586No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC191400.1125324125979154No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT184770.108634346268113No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC183340.10779358686364582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT48700.057.934766
GACCGCG50800.055.6428225
CGCGTTC52300.054.1361928
CCGCGTT52500.053.741397
AGACCGC54450.051.9128574
CAGACCG63800.044.460123
GCGTTCT71950.039.6952329
AATACGG63550.025.19718732-33
GAGTCTT218200.024.8652324
TGGAGTC229350.024.3039932
CAATACG59800.024.21129430-31
GGAGTCT230450.024.2093963
AACGATC65550.024.20185546-47
CTCGCTA180300.023.9721851
AGTCTTG237400.023.3963935
GAACGAT62100.023.3145844-45
TACGGAG49800.023.30809634-35
GTCTTGG239700.023.169586
TCGCTAT191750.022.4864462
GCAACGC65400.022.21544580-81
Produced by FastQC (version 0.11.5)