FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17008433
Sequences flagged as poor quality0
Sequence length105
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG385180.22646413105781113No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT347930.20456323048690023No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA256310.1506958342370517No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG254870.14984919539618966No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA249430.1466507819973774No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC246710.1450515752979713No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC236840.13924857157622927No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG232700.13681448490875087No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC217130.12766020244192983No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA209310.12306248318113726No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT208110.12235695081375221No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG203860.11985819034593018No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG188790.11099787969885291No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG178190.10476567712028498No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT170150.10003861025880513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC116150.037.364351
GGTGGCG151350.028.3458022
GGCGATC28650.026.8015792
GCGACAT66600.025.4327167
GGGCGAT33600.024.6234951
GGCGCGT175350.024.291985
TGCGACA68550.024.2035036
TGGCGCG182300.023.5301384
GCGCGTG201500.021.1871786
CTGCGAC79100.021.0393855
GCTGCGA81550.020.7120784
CGACATC85050.020.2042928
GTGGCGC212900.020.1495863
CGCGTGC230250.018.6061847
CGATCTG46600.017.751044
GCGTGCC256050.016.7293788
GGCTGCG101950.016.665983
GCGATCT49000.016.2762263
CGTGCCT298000.014.4901249
GCCACGA66050.013.869554518-19
Produced by FastQC (version 0.11.5)