FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6832034
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT144230.2111084341793381No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA85740.12549703353349823No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC68770.10065816417189961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT15100.057.2544026
GACCGCG15750.055.1931235
CGCGTTC16150.053.529248
CCGCGTT16550.052.235487
AGACCGC17650.048.982524
CAGACCG23400.036.9462173
GCGTTCT23450.036.865559
TACGGAG20850.024.14860234-35
AATACGG22050.023.04981232-33
CCGTACG4950.022.0717664
CGTACGC5050.021.6347035
CAACGCC24550.020.60583982-83
ATACGGA21600.020.56127532-33
AACGATC25000.020.51993446-47
CAATACG21800.020.3726430-31
GTCCGTT6100.020.2468741
GCAACGC21250.020.22934780-81
AGAACGA25900.019.89858444-45
CGGAGAG26500.019.53767236-37
CGATCAT26850.019.28299148-49
Produced by FastQC (version 0.11.5)