FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6832034
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72500.10611773887542128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC8200.027.8034692
GGGCGAT11100.022.2504731
GGCGCGT7800.021.3163074
CGATCTG12700.019.0767774
TGCGACA15950.016.979176
GCGCGTG10400.016.4455785
CGCGTGC10600.015.6878886
GCGACAT16500.015.5495747
CTGCGAC18350.014.49879555
ACGTGCG9600.013.36378
GCGTACG2002.0443622E-613.03853694-95
GCTGCGA20900.012.9558854
GCGATCT18200.012.7889393
GCGTCCT16800.011.88565232-33
CGTGCGC10550.011.7108119
CCGGTAG6906.657501E-911.0174117
TGGCGCG15100.011.0103453
GCCACGA16900.010.82868318-19
GAGTGCG17150.010.81231228-29
CGACATC25050.010.8118828
Produced by FastQC (version 0.11.5)